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The Bam Complex: Methods and Protocols

The Bam Complex: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 1329

BiologyChemistry

ISBN10: 1493949209
ISBN13: 9781493949205
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 283
Weight: 1.14
Height: 0.62 Width: 7.00 Depth: 10.00
Language: English

This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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