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Difference Gel Electrophoresis: Methods and Protocols

Difference Gel Electrophoresis: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 1664

BiologyChemistry

Currently unavailable to order

ISBN10: 1493984365
ISBN13: 9781493984367
Publisher: Humana
Published: Aug 22 2018
Pages: 318
Language: English

This volume provides a comprehensive study of this key method of gel-based proteomics. Chapters in Difference Gel Electrophoresis: Methods and Protocols detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced mass spectrometry for protein identification, application of basic biological research, and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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