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Drosophila: Methods and Protocols

Drosophila: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 2540

BiologyGeneral ScienceZoology

ISBN10: 1071625438
ISBN13: 9781071625439
Publisher: Humana
Published: Aug 20 2023
Pages: 424
Weight: 1.66
Height: 0.89 Width: 7.00 Depth: 10.00
Language: English
This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls

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Dahmann, Christian

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