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Flavins and Flavoproteins: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2280
ISBN13: 9781071612880
Publisher: Springer Nature
Published: Mar 23 2022
Pages: 298
Weight: 1.21
Height: 0.66 Width: 7.00 Depth: 10.00
Language: English
Part I: Riboflavin Production and Export by Microorganisms
1. Selection of Riboflavin Overproducing Strains of Lactic Acid Bacteria and Riboflavin Direct Quantification by Fluorescence
Pasquale Russo, Nicola De Simone, Vittorio Capozzi, Mari Luz Mohedano, José Ángel Ruiz-Masó, Gloria Del Solar, Paloma López, and Giuseppe Spano
2. Recent Advances in Construction of the Efficient Producers of Riboflavin and Flavin Nucleotides (FMN, FAD) in the Yeast Candida Famata
Dariya V. Fedorovych, Kostyantyn V. Dmytruk, and Andriy A. Sibirny
3. Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida Famata
Andriy O. Tsyrulnyk, Dariya V. Fedorovych, Kostyantyn V. Dmytruk, and Andriy A. Sibirny
Part II: Measuring Riboflavin Transport and Flavin Cofactor Metabolism with Recombinant Human Proteins
4. Functional Study of the Human Riboflavin Transporter 2 Using Proteoliposomes System
Lara Console, Maria Tolomeo, and Cesare Indiveri
5. Heterologous Over-Expression of Human FAD Synthase Isoforms 1 and 2
Michele Galluccio and Cesare Indiveri
6. Purification of Recombinant Human 6His-FAD Synthase (Isoform 2) and Quantitation of FAD / Protein Monomer Ratio by UV/Vis Spectra
Piero Leone, Stefano Quarta, Maria Tolomeo, and Maria Barile
7. Continuous and Discontinuous Approaches to Study FAD Synthesis and Degradation Catalyzed by Purified Recombinant FAD Synthase or Cellular Fractions
Piero Leone, Maria Tolomeo, and Maria Barile
Part III: Looking at Flavin Cofactors in Flavoenzymes
8. Redox Titration of Flavoproteins: An Overview
Francesco Bonomi and Stefania Iametti
9. Anaerobic Stopped-Flow Spectrophotometry with Photodiode Array Detection in the Pre-Steady State: An Application to Elucidate Oxido-Reduction Mechanisms in Flavoproteins
Patricia Ferreira and Milagros Medina
10. Atomic Force Microscopy: Single Molecule Imaging and Force Spectroscopy in the Study of Flavoproteins Ligand Binding and Reaction Mechanisms
Anabel Lostao and Milagros Medina
11. Ligand Binding in Allosteric Flavoproteins: Part 1. Quantitative Analysis of the Interaction with NAD+ of the Apoptosis Inducing Factor (AIF) Harboring FAD in the Reduced State
Paolo Cocomazzi, Luca Sorrentino, Federica Cossu, and Alessandro Aliverti
12. Ligand Binding in Allosteric Flavoproteins: Part 2. Quantitative Analysis of the Redox-Dependent Interaction of the Apoptosis-Inducing Factor (AIF) with Its Protein Partner
Paolo Cocomazzi, Delia Tarantino, Eloise Mastrangelo, and Alessandro Aliverti
13. Using D- and L-Amino Acid Oxidases to Generate the Imino Acid Substrate to Measure the Activity
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