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Protein Blotting and Detection: Methods and Protocols

Protein Blotting and Detection: Methods and Protocols

Paperback

Series: Methods in Molecular Biology, Book 536

BiologyChemistry

Currently unavailable to order

ISBN10: 1617379433
ISBN13: 9781617379437
Publisher: Humana
Published: Jan 26 2011
Pages: 588
Language: English

Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In Protein Blotting and Detection: Methods and Protocols, expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting both adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

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