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Intrinsically Disordered Proteins: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 2141
ISBN13: 9781071605264
Publisher: Humana
Published: Jul 23 2021
Pages: 951
Weight: 3.63
Height: 1.91 Width: 7.00 Depth: 10.00
Language: English
Part I: Sequence Properties
1. Disorder for Dummies: Functional Mutagenesis of Transient Helical Segments in Disordered Proteins
Gary W. Daughdrill
2. Computational Prediction of Intrinsic Disorder in Protein Sequences with the disCoP Meta-predictor
Christopher J. Oldfield, Xiao Fan, Chen Wang, A. Keith Dunker, and Lukasz Kurgan
3. Computational Prediction of Disordered Protein Motifs using SLiMSuite
Richard J Edwards, Kirsti Paulsen, Carla M Aguilar Gomez, and Åsa Pérez-Bercoff
4. How to Annotate and Submit a Short Linear Motif to the Eukaryotic Linear Motif Resource
Marc Gouw, Jesús AlvaradoValverde, Jelena Čalyseva, Francesca -------Diella, Manjeet Kumar, Sushama Michael, Kim Van Roey, Holger Dinkel, and Toby J. Gibson
5. Analyzing the Sequences of Intrinsically Disordered Regions with CIDER and localCIDER
Garrett M. Ginell and Alex S. Holehouse
6. Exploring Protein Intrinsic Disorder with MobiDB
Alexander Miguel Monzon, András Hatos, Marco Necci, Damiano Piovesan, and Silvio C. E. Tosatto
Part II: Evolution
7. An Easy Protocol for Evolutionary Analysis of Intrinsically Disordered Proteins
Janelle Nunez-Castilla and Jessica Siltberg-Liberles
Part III: Production
8. Expression and Purification of an Intrinsically Disordered Protein
Karamjeet K. Singh and Steffen P. Graether
9. Production of Intrinsically Disordered Proteins for Biophysical Studies; Tips and Tricks
Christian Parsb k Pedersen, Pernille Seiffert, Inna Brakti, and Katrine Bugge
10. Recombinant Production of Monomeric Isotope-Enriched Aggregation-Prone Peptides: Polyglutamine Tracts and Beyond
Albert Escobedo, Giulio Chiesa, and Xavier Salvatella
11. Cell-free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopy
Linnéa Isaksson and Anders Pedersen
Part IV: Dynamics, Ensembles, and Structures
12. Structural Analyses of Intrinsically Disordered Proteins by Small-Angle X-ray Scattering
Amin Sagar, Dmitri Svergun, and Pau Bernadó
13. Determining Rg of IDPs from SAXS data
Ellen Rieloff and Marie Skepö
14. Obtaining Hydrodynamic Radii of Intrinsically Disordered Protein Ensembles by Pulsed Field Gradient NMR Measurements
Sarah Leeb and Jens Danielsson
15. Quantitative Protein Disorder Assessment using NMR Chemical Shifts
Jakob T. Nielsen and Frans A. A. Mulder
16. Determination of pKa Values in Intrinsically Disordered Proteins
Brandon Payliss and Anthony Mittermaier
17. Paris-DÉCOR: A Protocol for the Determination of Fast Protein Backbone Amide Hydrogen Exchange Rates
Rupashree Dass and Frans A.A. Mulder
Part V: Ensembles by Computation
18. Predicting Conformational Properties of Intrinsically Disordered Proteins from Sequence
Kiersten M. Ruff
19. Enhanced Molecular Dynamics Simulations of Intrinsically Disordered Proteins
Matteo Masetti, Mattia Bernetti, and Andrea Cavalli
20. Computational Protocol for Determining Conformational Ensembles of Intrinsically Disordered Proteins
Robert B. Best
21. Computing, Analyzing and Comparing the Radius of Gyration and Hydrodynamic Radius in Confo
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