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Strain Engineering: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 765
Medical ReferenceTechnology & EngineeringGenetics
ISBN13: 9781493958467
Publisher: Humana
Published: Aug 23 2016
Pages: 480
Weight: 1.88
Height: 1.00 Width: 7.00 Depth: 10.00
Language: English
Part 1. E. coli
1. Bacterial Genome Reengineering
Jindan Zhou and Kenneth E. Rudd
2. Targeted Chromosomal Gene Knockout using PCR Fragments
Kenan C. Murphy
3. Scarless Chromosomal Gene Knockout Methods
Bong Hyun Sung, Jun Hyoung Lee, and Sun Chang Kim
4. Random Chromosomal Gene Disruption In vivo using Transposomes
Les M. Hoffman
5. Genome Engineering using Targeted Oligonucleotide Libraries and Functional Selection
Elie J. Diner, Fernando Garza-Sánchez, and Christopher S. Hayes
6. Microarray-based Genetic Footprinting Strategy to Identify Strain Improvement Genes after Competitive Selection of Transposon Libraries
Alison K. Hottes and Saeed Tavazoie
7. Optimization of Synthetic Operons using Libraries of Post-Transciptional Regulatory Elements
Daniel E. Agnew and Brian F. Pfleger
8. Marker Free Chromosomal Expression of Foreign and Native Genes in E. coli
Chung-Jen Chiang, Po Ting Chen, Shan-Yu Chen, and Yun-Peng Chao
9. Array-based Synthetic Genetic Screens to Map Bacterial Pathways and Functional Networks in Escherichia coli
Mohan Babu, Alla Gagarinova, and Andrew Emili
10. Assembling New E. coli Strains by Transduction using Phage P1
Sean D. Moore
Part II. Saccharomyces cerevisiae
11. Yeast Bioinformatics and Strain Engineering Resources
Audrey L. Atkin
12. Delete and Repeat: A Comprehensive Toolkit for Sequential Gene Knock-out in the Budding Yeast Saccharomyces cerevisiae
Johannes H. Hegemann and Sven Boris Heick
13. Genome-wide Transposon Mutagenesis in Saccharomyces cerevisiae and Candida albicans
Tao Xu, Nikë Bharucha, and Anuj Kumar
14. Signature-tagged Mutagenesis to Characterize Genes Through Competitive Selection of Barcoded Genome Libraries
Julia Oh and Corey Nislow
15. Global Strain Engineering by Mutant Transcription Factors
Amanda M. Lanza and Hal S. Alper
16. Genomic Promoter Replacement Cassettes to Alter Gene Expression in the Yeast Saccharomyces cerevisiae
Andreas Kaufmann and Michael Knop
Part III. Strain Engineering Other Industrially Important Microbes
17. Microbial Genome Analysis and Comparisons: Web-based Protocols and Resources
Medha Bhagwat and Arvind A. Bhagwat
18. Plasmid Artificial Modification: A Novel Method for Efficient DNA Transfer into Bacteria
Tohru Suzuki and Kazumasa Yasui
19. Broad-host-range Plasmid Vectors for Gene Expression in Bacteria
Rahmi Lale, Trygve Brautaset, and Svein Valla
20. Simple Method for Introducing Marker-free Deletions in the Bacillus subtilis Genome
Takuya Morimoto, Katsutoshi Ara, Katsuya Ozaki, and Naotake Ogasawara
21. Transposon-mediated Random Mutagenesis of Bacillus subtilis
Adam C. Wilson and Hendrik Szurmant
22. Integrative Food Grade Expression System for Lactic Acid Bacteria
Grace L. Douglas, Yong Jun Goh and Todd R. Klaenhammer
23. ClosTron-mediated Engineering of Clostridium
Sarah A. Kuehne, John T. Heap, Clare M. Cooksley, Stephen T. Cartman, and Nigel P. Minton
24. High Throughput Transposon Mutagenesis of Corynebacterium glutamicum
Nobuaki Suzuki, Masayuki Inui, and Hideaki Yukawa
25. Mini-Mu Transposon Mutagenesis of Ethanologenic Zymomonas mobilis
Katherine M. Pappas
26. Engineering Thermoacidop
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