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Bioconjugation Protocols: Strategies and Methods

Bioconjugation Protocols: Strategies and Methods

Paperback

Series: Methods in Molecular Biology, Book 751

Medical ReferenceBiologyChemistry

ISBN10: 1493960962
ISBN13: 9781493960965
Publisher: Humana Pr
Published: Aug 23 2016
Pages: 605
Weight: 2.35
Height: 1.26 Width: 7.00 Depth: 10.00
Language: English

Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology. In this second edition of Bioconjugation Protocols: Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering. These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches. Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices). All the protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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