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Test topnienia o wysokiej rozdzielczości do wykrywania infekcji bakteryjnych STD
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ISBN10: 6208728150
ISBN13: 9786208728151
Publisher: Wydawnictwo Nasza Wiedza
Published: Mar 11 2025
Pages: 108
Weight: 0.34
Height: 0.26 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786208728151
Publisher: Wydawnictwo Nasza Wiedza
Published: Mar 11 2025
Pages: 108
Weight: 0.34
Height: 0.26 Width: 6.00 Depth: 9.00
Language: Polish
Obecne badanie zostalo przeprowadzone w celu wykrycia czynników zakaźnych przenoszonych drogą plciową (bakterii i wirusów) poprzez opracowanie testu HRM (topnienie w wysokiej rozdzielczości) z multipleksowym PCR w czasie rzeczywistym przy użyciu zestawu starterów każdego patogenu, który zostal wyznaczony przez profesjonalne oprogramowanie (Beacon Designer (TM) automatyzuje projektowanie starterów i sond w czasie rzeczywistym) w celu dokladnego wykrycia tych infekcji u pacjentów z Iraku. Badanie to przeprowadzono w celu wykrycia siedmiu różnych patogenów przenoszonych drogą plciową (3 bakterii, 3 wirusów i jednego pasożyta) wśród 150 podejrzanych pacjentów jako par malżeńskich. Wyniki RT-PCR z testem HRM kontroli pozytywnych wykazaly różne krzywe topnienia, gdzie każdy pik topnienia reprezentuje amplifikację jednego z siedmiu patogenów, a także pik topnienia kontroli wewnętrznej (gen podtrzymujący). Eksperyment ten obejmowal trzy panele, panel pierwszy reprezentuje optymalny pik topnienia dla pozytywnej kontroli Trichomonas vaginalis, Mycoplasma hominis, Cytomegalovirus, temperatura topnienia każdego z nich wynosila odpowiednio 80,0 ℃, 84,90 ℃ i 88,0 ℃.
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