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Wzór pigmentu podczas stadiów embrionalnych u heteroploidalnego Bufo regularis
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ISBN10: 6208660149
ISBN13: 9786208660147
Publisher: Wydawnictwo Nasza Wiedza
Published: Feb 18 2025
Pages: 92
Weight: 0.30
Height: 0.22 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786208660147
Publisher: Wydawnictwo Nasza Wiedza
Published: Feb 18 2025
Pages: 92
Weight: 0.30
Height: 0.22 Width: 6.00 Depth: 9.00
Language: Polish
W niniejszej publikacji wykorzystano stadia larwalne ropuchy egipskiej Bufo regularis (Bufonidae). Analizie poddano trzy grupy nowo zaplodnionych jaj. Jedną grupę napromieniowano ultrafioletem (tj. haploidalną), drugą poddano szokowi termicznemu (tj. triploidalną), a trzecią wykorzystano jako kontrolę (tj. diploidalną). Przekroje poprzeczne diploidalnych, haploidalnych i triploidalnych zarodków w różnych stadiach embrionalnych wykorzystano do zbadania rozmieszczenia centrów melano-makrofagów (MMC). Komórki barwnikowe i MMC wykryto za pomocą mikroskopii świetlnej w wątrobie, nerce i śledzionie trzech badanych grup kijanek B. regularis. Co więcej, scharakteryzowano również MMCs w wątrobie, nerce i śledzionie w różnych stadiach embrionalnych B. regularis. Z drugiej strony, przy użyciu wysoce czulego testu odwrotnej transkrypcji - reakcji lańcuchowej polimerazy (RT-PCR) wykryto mRNA tyrozynazy w wątrobie i śledzionie trzech badanych grup kijanek B. regularis; dlatego komórki pigmentowe wątroby i śledziony (KC) są zdolne do syntezy melaniny. mRNA tyrozynazy nie ulega ekspresji w nerce; więc komórki pigmentowe nerki są makrofagami fagocytującymi melaninę.
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