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Peptide Synthesis and Applications

Peptide Synthesis and Applications

Paperback

Series: Methods in Molecular Biology, Book 298

Medical ReferenceChemistry

ISBN10: 1617374903
ISBN13: 9781617374906
Publisher: Springer Nature
Published: Nov 19 2010
Pages: 262
Weight: 0.89
Height: 0.62 Width: 6.00 Depth: 9.00
Language: English
Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.

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