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Receptor Signal Transduction Protocols: Third Edition
Paperback
Series: Methods in Molecular Biology, Book 746
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ISBN13: 9781493956258
Publisher: Humana
Published: Aug 23 2016
Pages: 479
Weight: 1.86
Height: 0.99 Width: 7.01 Depth: 10.00
Language: English
Part I: Developing Models for Studying GPCRs
1. Inducible Expression of G Protein-Coupled Receptors in Transfected Cells
Beryl Koener and Emmanuel Hermans
2. Using the Flp-InTM T-RexTM System to Regulate GPCR Expression
Richard J. Ward, Elisa Alvarez-Curto, and Graeme Milligan
3. Viral Infection for GPCR Expression in Eukaryotic Cells
Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi
4. Generation of Epitope-Tagged GPCRs
Yan Huang and Gary B. Willars
5. The Use of Site-Directed Mutagenesis to Study GPCRs
Alex C. Conner, James Barwell, David R. Poyner, and Mark Wheatley
6. Approaches to Study GPCR Regulation in Native Systems
Jonathon M. Willets
7. Heterologous Expression of GPCRs in Fission Yeast
John Davey and Graham Ladds
Part II: Examining GPCR Expression and Agonist-Induced Covalent Modifications
8. Radioligand Binding Methods for Membrane Preparations and Intact Cells
David B. Bylund and Myron L. Toews
9. Quantification of GPCR mRNA Using Real-Time RT-PCR
Trond Brattelid and Finn Olav Levy
10. Determining Allosteric Modulator Mechanism of Action: Integration of Radioligand Binding and Functional Assay Data
Christopher J. Langmead
11. Design and Use of Fluorescent Ligands to Study Ligand-Receptor Interactions in Single Living Cells
Stephen J. Briddon, Barrie Kellam, and Stephen J. Hill
12. Examining Site-Specific GPCR Phosphorylation
Adrian J. Butcher, Andrew B. Tobin, and Kok Choi Kong
13. Ubiquitination of GPCRs
Adriana Caballero and Adriano Marchese
Part III: Examining Early Events in GPCR Signaling
14. [35S]GTPγS Binding as an Index of Total G-Protein and Gα-Subtype-Specific Activation by GPCRs
Rajendra Mistry, Mark R. Dowling, and R.A. John Challiss
15. Using Calcium Imaging as a Readout of GPCR Activation
Martin D. Bootman and H. Llewelyn Roderick
16. Measuring Spatio-Temporal Dynamics of Cyclic AMP Signaling in Real-Time Using FRET-Based Biosensors
Frank Gesellchen, Alessandra Stangherlin, Nicoletta Surdo, Anna Terrin, Anna Zoccarato, and Manuela Zaccolo
17. Determining the Activation of Rho as an Index of Receptor Coupling to G12/13 Proteins
Michio Nakaya, Mina Ohba, Motohiro Nishida, and Hitoshi Kurose
18. The Use of Translocating Fluorescent Biosensors for Real-Time Monitoring of GPCR-Mediated Signaling Events
Carl P. Nelson and R.A. John Challiss
Part IV: Receptor-Receptor and Receptor-Protein Interactions
19. Study of GPCR-Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Protein Pull-Downs
Ashley E. Brady, Yunjia Chen, Lee E. Limbird, and Qin Wang
20. Study of GPCR-Protein Interactions by BRET
Martina Kocan and Kevin D.G. Pfleger
21. Time Resolved FRET Strategy with Fluorescent Ligands to Analyze Receptor Interactions in Native Tissues: Application to GPCR Oligomerization
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