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Signal Transduction Immunohistochemistry: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 717
ISBN13: 9781617790232
Publisher: Springer Nature
Published: Feb 14 2011
Pages: 302
Weight: 1.60
Height: 0.90 Width: 7.10 Depth: 10.10
Language: English
Part I: Antibodies as a Tool: From Concept to Design and Application
1. Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies
Kathy Brumbaugh, Wade Johnson, Wen-Chieh Liao, Mong-Shang Lin, J. P. Houchins, Jeff Cooper, Steven Stoesz, and Roberto Campos-Gonzalez
2. Selection and Validation of Antibodies for Signal Transduction Immunohistochemistry
Juraj Bodo and Eric D. His
3. An Overview of Western Blotting for Determining Antibody Specificities for Immunohistochemistry
Biji T. Kurien, Yaser Dorri, Skyler Dillon, Anil Dsouza, and R. Hal Scofield
4. Optimized Protocol to Make Phospho-Specific Antibodies that Work
Amy J. Archuleta, Crystal A. Stutzke, Kristin M. Nixon, and Michael D. Browning
Part II: Preservation and Unmasking of Tissue Antigens
5. Methodology and Technology for Stabilization of Specific States of Signal Transduction Proteins
Mats Borén
6. An Enhanced Antigen Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-Embedded Tissues
Sergei I. Syrbu and Michael B. Cohen
Part III: Imaging Techniques and High-Throughput Data Analysis
7. Imaging Techniques in Signal Transduction IHC
Jerry Sedgewick
8. Practical Considerations of Image Analysis and Quantification of Signal Transduction IHC Staining
Michael Grunkin, Jakob Raundahl, and Niels T. Foged
9. Flow Cytometric Analysis of Cell Signaling Proteins
Maria A. Suni and Vernon C. Maino
10. CytoSys: A Tool for Extracting Cell Cycle-Related Expression Dynamics from Static Data
Jayant Avva, Michael C. Weis, Radina P. Soebiyanto, James W. Jacobberger, and Sree N. Sreenath
Part IV: Neuroscience, Cancer, and Stem Cell Research
11. Signaling Events Initiated by Kappa Opioid Receptor Activation: Quantification and Immunolocalization Using Phospho-Selective KOR, p38-MAPK, and KIR 3.1 Antibodies
Julia C. Lemos, Clarisse Roth, and Charles Chavkin
12. Immunohistochemical Assessment of Signal Transduction and Cell-Cycle Networks in Neural Tumors
Daniel Ciznadija, Afsar Barlas, and Katia Manova
13. Novel Multi-Color Immunofluorescence Technique Using Primary Antibodies Raised in the Same Host Species
Jillian Frisch, J.P. Houchins, Michael Grahek, Jordan Schoephoerster, Jodi Hagen, Joseph Sweet, Leopoldo Mendoza, David Schwartz, and Alexander E. Kalyuzhny
14. Activation and Differentiation of Mesenchymal Stem Cells
Pravin J. Mishra and Debabrata Banerjee
Part V: Novel Assays and Techniques
15. Double In situ Detection of Sonic Hedgehog mRNA and pMAPK Protein in Examining the Cell Proliferation Signaling Pathway in Mouse Embryo
Sho Fujisawa, Mesruh Turkekul, Afsar Barlas, Ning Fan, and Katia Manova
16. Identifying Intracellular Sites of Eicosanoid Lipid Mediator Synthesis with EicosaCell Assays
Christianne Bandeira-Melo1, Peter F. Weller2, and Patricia T. BozzaChristianne Bandeira-Melo, Peter F. Weller, and Patricia T. Bozza
17. Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
Jordan Schoephoerster, Jil
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