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Therapeutic Oligonucleotides: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 764
Technology & EngineeringChemistry
ISBN13: 9781617791871
Publisher: Humana
Published: Jul 15 2011
Pages: 340
Weight: 1.83
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English
1. Therapeutic Oligonucleotides
John Goodchild
2. Dinucleotides Containing 3[-S-Phosphorothiolate Linkages
James W. Gaynor and Richard Cosstick
3. 2'-O,4'-C-Methyleneoxymethylene Bridged Nucleic Acids (2',4'-BNACOC)
Yoshiyuki Hari, Tetsuya Kodama, Takeshi Imanishi, and Satoshi Obika
4. A Non-covalent Peptide-based Strategy for Ex-vivo and In vivo Oligonucleotide Delivery
Laurence Crombez, May C. Morris, Frederic Heitz, and Gilles Divita
5. Cell Penetrating Peptides-based Strategies for the Delivery of Splice Redirecting Antisense Oligonucleotides
Samir El Andaloussi, Fatouma Said Hassane, Prisca Boisguerin, Rannar Sillard, Ulo Langel, and Bernard Lebleu
6. A Nanoparticle for Tumor Targeted Delivery of Oligomers
Xinrong Liu, Yi Wang, and Donald J. Hnatowich
7. Light-directed Delivery of Nucleic Acids
Sigurd B e, Lina Prasmickaite, Birgit Enges ter, and Eivind Hovig
8. Antibody Targeted siRNA Delivery
Masoud M. Toloue and Lance P. Ford
9. Aptamer-Drug Conjugation for Targeted Tumor Cell Therapy
Michael J. Donovan, Ling Meng, Tao Chen, Yunfei Zhang, Kwame Sefah, and Weihong Tan
10. Five-step Process for Screening Antisense Compounds for Efficacy: Gene Target IL-12Rb2
Nikki B. Marshall, Laura L. Hauck, and Dan V. Mourich
11. Diverse Small Non-coding RNAs in RNA Interference Pathways
Liande Li and Yi Liu
12. Quantification of siRNAs In vitro and In vivo
Angie Cheng, Alexander V. Vlassov, and Susan Magdaleno
13. Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR
Angie Cheng, Susan Magdaleno, and Alexander V. Vlassov
14. siRNA Knockdown of Gene Expression in Endothelial Cells
Emily Dennstedt and Brad Bryan
15. Using RNA Interference in Schistosoma mansoni
Rita Bhardwaj, Greice Krautz-Peterson, and Patrick J. Skelly
16. Performing the Labeled microRNA Pull-down (LAMP) Assay System: An Experimental Approach for High-throughput Identification of microRNA-target mRNAs
Ren-Jun Hsu and Huai-Jen Tsai
17. Synthesis, Purification, and Characterization of Oligoribonucleotides that Act as Agonists of TLR7 and/or TLR8
Tao Lan and Ekambar R. Kandimalla
18. Synthesis, Purification, and Characterization of Immune-Modulatory Oligodeoxynucleotides that Act as Agonists of Toll-like Receptor 9
Mallikarjuna Reddy Putta, Dong Yu, and Ekambar R. Kandimalla
19. Surface Plasmon Resonance Investigation of RNA Aptamer-RNA Ligand Interactions
Carmelo Di Primo, Eric Dausse, and Jean-Jacques Toulmé
20. Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute Protein
Jacob C. Schwartz and David R. Corey
21. Inhibition of Human Papillomavirus Expression Using DNAzymes
MarÃa Luisa BenÃtez-Hess, Pablo Reyes-Gutiérrez, and Luis Marat Alvarez-Salas
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