Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Application of X-Ray Cryo-Tomography Technique (Cryo-XT)

Application of X-Ray Cryo-Tomography Technique (Cryo-XT)

Moreno Serrano, Jose Antonio
Morocho Chamba, Wagner Roberto
Jimenez Ávila, Luis Antonio

Paperback

Biology

ISBN10: 6207701151
ISBN13: 9786207701155
Publisher: Our Knowledge Publishing
Published: Jun 26 2024
Pages: 52
Weight: 0.20
Height: 0.12 Width: 6.00 Depth: 9.00
Language: English
VV (vaccinia virus) is one of the most complex viruses, with a size of more than 300 nm and more than 100 structural proteins. Its assembly involves sequential interactions and major rearrangements of its structural components. In this study, infected cells were selected by light fluorescence microscopy and subsequently imaged by X-ray microscopy under cryogenic conditions. Tomographic tilt series of X-ray images were used to produce three-dimensional reconstructions showing different cellular organelles (nuclei, mitochondria, ER), along with two other types of viral particles related to different stages of vaccinia virus maturation (IV) immature and (MV) mature particles; witaferin assays showed actin binding, which prevents polymerization and elongation of filaments; causing mispackaged or aberrant virions, which inhibits the progression of viral infection. The findings demonstrate that X-ray cryo-tomography is a powerful tool for collecting three-dimensional structural information from frozen, unfixed whole cells.

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