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Bacterial Cell Wall Homeostasis: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 1440
ISBN13: 9781493981144
Publisher: Humana Pr
Published: May 30 2018
Pages: 288
Weight: 1.16
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English
Part I Analyzing Physical Properties of the Bacterial Cell Wall
1. Atomic Force Microscopic Analysis of Bacterial Cell Wall Peptidoglycan Architecture Robert D. Turner, Jamie K. Hobbs, and Simon J. Foster
2. Ultra-Sensitive High Throughput Analytical Methods to Identify Novel Components Involved in Cell Wall Biology and to Study Bacterial Cell Wall Dynamics
Part II Genome-Wide Approaches for the Identification of Gene Products with Roles in Cell Wall Homeostasis
3. Microarray Analysis to Monitor Bacterial Cell Wall Homeostasis
Hee-Jeon Hong and Andy Hesketh
4. Cell Shaving and False Positive Control Strategies Coupled to Novel Statistical Tools to Profile Gram-Positive Bacterial Surface ProteomesNestor Solis and Stuart J. Cordwell
5. Differential Proteomics Based on Multidimensional Protein Identification Technology to Understand the Biogenesis of Outer Membrane of Escherichia coliAlessandra M. Martorana, Sara Motta, Paola Sperandeo, Pierluigi Mauri, and Alessandra Polissi
6. Random Transposon Mutagenesis for Cell-Envelope Resistant to Phage Infection Ruth Reyes-Cortés, Emma S. Arguijo-Hernández, Marco A. Carballo-Ontiveros, Eva MartÃnez-Peñafiel and Luis Kameyama
Part III Functional Analysis of Cell-Wall Associated Proteins
7. Zymographic Techniques for the Analysis of Bacterial Cell Wall in Bactillus
Tatsuya Fukushima and Junichi Sekiguchi
8. Liquid Chromatography-Tandem Mass Spectrometry to Define Sortase Cleavage Products
Andrew Duong, Kalinka Koteva, Danielle L. Sexton, and Marie A. Elliot
9. Genetics and Cell Morphology Analyses of the Actinomyces oris srtA Mutant
Chenggang Wu, Melissa Elizabeth Reardon-Robinson, and Hung Ton-That
Part IV Reporter Assays for Cell Wall Stress
10. Construction of a Bioassay System to Identify Extracellular Agents Targeting Bacterial Cell Envelope
Hee-Jeon Hong
11. Luciferase Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus
Vanina Dengler and Nadine McCallum
Part V Analysis of the Non-Protein Components of the Cell Wall
12. Extraction and Analysis of Peptidoglycan Cell Wall Precursors
Elisa Binda, Lùcia Carrano, Giorgia Letizia Marcone, and Flavia Marinelli
Alexander J. F. Egan and Waldemar Vollmer
14. Analysis of Peptidoglycan Fragment Release
Ryan E. Schaub, Jonathan D. Lenz, and Joseph P. Dillard
Gonçalo Covas, Filipa Vaz, Gabriela Henriques, Mariana G. Pinho, and Sérgio R. Filipe
16. Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy
Madeleine R
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