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Études taxonomiques sur Phlebotomus (Larroussius) orientalis au Soudan
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ISBN10: 6209370535
ISBN13: 9786209370533
Publisher: Editions Notre Savoir
Published: Nov 27 2025
Pages: 108
Weight: 0.34
Height: 0.26 Width: 6.00 Depth: 9.00
Language: French
ISBN13: 9786209370533
Publisher: Editions Notre Savoir
Published: Nov 27 2025
Pages: 108
Weight: 0.34
Height: 0.26 Width: 6.00 Depth: 9.00
Language: French
Cette recherche a été menée afin d'étudier les différences morphométriques et moléculaires entre les populations de Phlebotomus orientalis provenant de trois régions différentes du Soudan. Des échantillons de phlébotomes ont été prélevés dans les États de Gedaref, du Nil Blanc et de Khartoum. Les spécimens de P. orientalis ont été collectés à l'aide de pièges lumineux miniatures CDC et de pièges à papier collant. Les spécimens de phlébotomes ont ensuite été montés sur des lames de verre sur un milieu de Brelese et identifiés au niveau de l'espèce. Les échantillons montés de P. orientalis ont ensuite été traités pour des mesures morphométriques de 23 et 29 caractères pour les femelles et les mâles respectivement. De plus, les gènes Cyt b, ITS2 rDNA 18S de P. orientalis provenant de différentes régions ont été amplifiés par PCR conventionnelle et en temps réel. Les échantillons amplifiés des gènes Cyt b et ITS2 ont été purifiés et séquencés. Les séquences ont été assemblées, éditées, analysées, puis comparées à la base de données GenBank. Les populations de P. orientalis provenant de trois zones géographiques ont montré un certain degré de différences sur la base d'analyses morphométriques et moléculaires.
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