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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Hydatidosis Diagnosis With Novel ELISA Kits

Hydatidosis Diagnosis With Novel ELISA Kits

Waleed Mustafa, Nisreen
Sadoon Shani, Wafaa

Paperback

Biology

ISBN10: 6207998278
ISBN13: 9786207998272
Publisher: LAP Lambert Academic Publishing
Published: Aug 12 2024
Pages: 112
Weight: 0.39
Height: 0.27 Width: 6.00 Depth: 9.00
Language: English
This study included five antigens, each examined with three immunoglobulins (total IgG, IgG1, and IgG4) using chequerboard titration ELISA to determine the optimal antigen concentration and serum dilutions for preparing diagnostic kits. The indirect ELISA method was then used to evaluate the diagnostic performance values (DPV) (sensitivity, specificity, diagnostic efficiency, positive predictive value, and negative predictive value) based on the cutoff value of each antigen with total IgG and its subclasses (IgG1 and IgG4). Antigen B (AgB) was purified from hydatid fluid and electrophoretically treated to isolate its bands (8 kDa, 16 kDa, and 24 kDa AgB), which were used as antigens, in addition to an antigen purified from protoscoleces. Among the 15 prepared kits, those containing the 8 kDa subunit of AgB and IgG4 showed the best performance. Results from the ROC curve indicated that the 8 kDa subunit had the highest diagnostic accuracy when tested with IgG4 and IgG1. Additionally, this study included the immunization of rabbits with AgB, and the results indicated that AgB can modulate the immune response toward a Th2 bias.

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