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Clonazione, espressione eterologa e purificazione parziale di IF2
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ISBN10: 6208845300
ISBN13: 9786208845308
Publisher: Edizioni Sapienza
Published: Jul 9 2025
Pages: 80
Weight: 0.26
Height: 0.19 Width: 6.00 Depth: 9.00
Language: Italian
ISBN13: 9786208845308
Publisher: Edizioni Sapienza
Published: Jul 9 2025
Pages: 80
Weight: 0.26
Height: 0.19 Width: 6.00 Depth: 9.00
Language: Italian
È importante valutare il ruolo dell'IF2 all'interno del meccanismo di regolazione della traduzione batterica, nonché proporre l'IF2 come bersaglio molecolare per farmaci antinfiammatori non steroidei (FANS) riproposti. L'obiettivo di questo progetto è identificare un nuovo ed efficace bersaglio molecolare endogeno terapeutico nel Mycobacterium tuberculosis. In questo studio, ho identificato, clonato, espresso e parzialmente purificato il nuovo potenziale bersaglio terapeutico endogeno per vari farmaci antinfiammatori non steroidei (FANS) dal ceppo H37Rv del Mycobacterium tuberculosis: il potenziale infB micobatterico, Rv2839c, che è il presunto fattore di inizio della traduzione proteica 2 (IF2) in alcune specie di micobatteri. Concludo che ciò potrebbe portarci alla scoperta del nuovo ruolo antitubercolare di alcuni vecchi FANS poco costosi e/o dei loro derivati come farmaci efficaci contro l'IF2 del Mycobacterium tuberculosis.
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