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Kompletny proces transferu genów za pośrednictwem Agrobacterium w tytoniu
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ISBN10: 6208944554
ISBN13: 9786208944551
Publisher: Wydawnictwo Nasza Wiedza
Published: Jun 17 2025
Pages: 72
Weight: 0.24
Height: 0.17 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786208944551
Publisher: Wydawnictwo Nasza Wiedza
Published: Jun 17 2025
Pages: 72
Weight: 0.24
Height: 0.17 Width: 6.00 Depth: 9.00
Language: Polish
Agrobacterium tumefaciens to niezwykly gatunek bakterii glebowej, która ma zdolnośc infekowania komórek roślinnych fragmentem swojego DNA. Transformacja roślin za pośrednictwem A. tumefaciens stala się najczęściej stosowaną metodą wprowadzania obcych genów do komórek roślinnych i późniejszej regeneracji roślin transgenicznych. Tytoń jest wysoką, liściastą rośliną jednoroczną należącą do rodziny psiankowatych. Wszystkie części rośliny z wyjątkiem nasion zawierają nikotynę, ale jej stężenie zależy od różnych czynników, takich jak gatunek, rodzaj gleby, uprawa lub warunki pogodowe. Aneksyny są rozpuszczalnymi, hydrofilowymi bialkami zdolnymi do wiązania się z ujemnie naladowaną bloną. Na podstawie wyników niniejszych badań stwierdzono, że transformacja tytoniu za pośrednictwem A. tumefaciens byla skuteczną metodą transferu genu aneksyny. Metodologia ta może byc również wykorzystana do komercyjnej produkcji transformowanego tytoniu odpornego na sól i suszę. Niewątpliwie opracowanie procedur transformacji opartych na transferze genów za pośrednictwem A. tumefaciens dla nowych gatunków o znaczeniu gospodarczym jest wskazane, a wyniki uzyskane w ostatnich latach wskazują na obiecującą przyszlośc.
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