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Związany z suszą gen czynnika transkrypcyjnego MYB z Brassica
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ISBN10: 620389382X
ISBN13: 9786203893823
Publisher: Wydawnictwo Nasza Wiedza
Published: Apr 22 2025
Pages: 108
Weight: 0.34
Height: 0.26 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786203893823
Publisher: Wydawnictwo Nasza Wiedza
Published: Apr 22 2025
Pages: 108
Weight: 0.34
Height: 0.26 Width: 6.00 Depth: 9.00
Language: Polish
Rośnie zainteresowanie wykorzystaniem genów regulatorowych jako skutecznego sposobu rozwoju genotypów odpornych na stres, ponieważ rośliny reagują poprzez modulację ekspresji genów prowadzącą do homeostazy komórkowej, detoksykacji i przywrócenia wzrostu. Identyfikacja nowych genów czynników transkrypcyjnych i ich roli w regulacji ekspresji genów indukowanych stresem pomoże w zrozumieniu szlaków sygnalowych prowadzących do tolerancji na stres. Inżynieria genetyczna jest atrakcyjna ze względu na jej potencjal do szybszej poprawy tolerancji na stres abiotyczny w porównaniu z tradycyjną hodowlą roślin. Rodzina genów Myb jest reprezentowana przez 190 genów u Arabidopsis, ale jak dotąd nie ma doniesień na temat genów u roślin kapustnych. Niniejsza książka zawiera pierwszy raport na temat izolacji i klonowania genu czynnika transkrypcyjnego MYB związanego z suszą z Brassica carinata przy użyciu metody RT-PCR. Zastosowano trzy zestawy starterów specyficznych dla genu Myb, a gen zostal pomyślnie sklonowany, scharakteryzowany i potwierdzony przez izolację plazmidowego DNA, PCR kolonii, trawienie RE. Opis ten będzie bardzo pomocny, ponieważ transformacja roślin przy użyciu zidentyfikowanych genów regulatorowych jest zachęcająca i przydatna do opracowania ulepszonych odmian odpornych na suszę.
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