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Fundamentos de Técnicas Moleculares: Do ADN à Genómica
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ISBN10: 6207911776
ISBN13: 9786207911776
Publisher: Edicoes Nosso Conhecimento
Published: Aug 4 2024
Pages: 76
Weight: 0.27
Height: 0.18 Width: 6.00 Depth: 9.00
Language: Portuguese
ISBN13: 9786207911776
Publisher: Edicoes Nosso Conhecimento
Published: Aug 4 2024
Pages: 76
Weight: 0.27
Height: 0.18 Width: 6.00 Depth: 9.00
Language: Portuguese
Este livro aprofunda as técnicas essenciais de biologia molecular que são fundamentais para a investigação e o diagnóstico médico contemporâneos. Abrange os princÃpios básicos da extração de material genético, o que facilita a análise genética, os estudos de expressão genética e as aplicações forenses. São fornecidas explicações pormenorizadas sobre os princÃpios e o funcionamento da reação em cadeia da polimerase (PCR) e suas variantes, incluindo PCR quantitativa, PCR de transcrição inversa, bem como PCR aninhada, multiplex, hotstart e outros tipos principais. As técnicas de blotting, como Southern, Northern e Western blots, são discutidas em pormenor pelo seu papel na deteção de ácidos nucleicos ou proteÃnas especÃficos. O livro também simplifica a tecnologia de edição de genes CRISPR. As técnicas de sequenciação são revistas de forma exaustiva, com uma comparação entre a sequenciação Sanger tradicional e as tecnologias de sequenciação de nova geração (NGS), como a sequenciação Ion Torrent, Pyrosequencing, Nanopore e Illumina. As suas aplicações na análise do genoma, no diagnóstico de doenças, na diversidade microbiana e nos estudos evolutivos são destacadas, constituindo um recurso indispensável para cientistas, investigadores e estudantes no domÃnio da biologia molecular.
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