Open Daily: 10am - 10pm | Alley door pickup 10am - 7pm 3038 Hennepin Ave Minneapolis, MN
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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Quantitative detection of equine babesiosis by molecular techniques

Quantitative detection of equine babesiosis by molecular techniques

Martins Porto, Raquel

Paperback

Biology

ISBN10: 6207012887
ISBN13: 9786207012886
Publisher: Our Knowledge Publishing
Published: Jan 3 2024
Pages: 52
Weight: 0.20
Height: 0.12 Width: 6.00 Depth: 9.00
Language: English
Equine babesiosis is one of the main parasitic diseases of horses and causes great damage to animal health, so detecting it as soon as possible makes treatment more successful. The aim of this book was to apply the real-time PCR technique (Q-PCR) to quantify equine Babesiosis using primers specific to the Babesia equi haemoparasite. The method used was the extraction of blood DNA from horses followed by amplification with specific primers for Babesia equi parasites. Two types of test were carried out: nested-PCR and Q-PCR to identify and quantify the parasite. Seven horses of the breed were analysed, three of which showed high levels of the parasite, although they had no clinical symptoms of the disease at the time of collection, but fell ill months later. This study opens up the prospect of using the Q-PCR technique to diagnose this equine pathology.

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