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Skeletal Muscle Regeneration in the Mouse: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 1460
ISBN13: 9781493938087
Publisher: Springer
Published: Aug 6 2016
Pages: 346
Weight: 1.85
Height: 0.81 Width: 7.00 Depth: 10.00
Language: English
Part I Injury Models
1. Eccentric Contraction-Induced Muscle Injury: Reproducible, Quantitative, Physiological Models to Impair Skeletal Muscle's Capacity to Generate Force
Jarrod A. Call and Dawn A. Lowe
2. Volumetric Muscle Loss
Gengyun Le, Dawn A. Lowe, and Michael Kyba
4. Synergist Ablation as a Rodent Model to Study Satellite Cell Dynamics in Adult Skeletal Muscle
Tyler J. Kirby, John J. McCarthy, Charlotte A. Peterson, and Christopher S. Fry
5. Inducing and Evaluating Skeletal Muscle Injury by Notexin and Barium Chloride
Matthew T. Tierney and Alessandra Sacco
Glynnis A. Garry, Marie Lue Antony, and Daniel J. Garry 7. Fibrosis-Inducing Strategies in Regenerating Dystrophic and Normal Skeletal Muscle
Patrizia Pessina and Pura Muñoz-Cánoves Part II Evaluating Progenitor Cells 8. Isolation, Cryosection, and Immunostaining of Skeletal Muscle
Huascar P. Ortuste Quiroga, Katsumasa Goto, and Peter S. Zammit 9. Isolation of Mouse Periocular Tissue for Histological and Immunostaining Analyses of the Extraocular Muscles and their Satellite Cells
Pascal Stuelsatz and Zipora Yablonka-Reuveni 10. Skeletal Muscle Tissue Clearing for LacZ and Fluorescent Reporters, and Immunofluorescence Staining
Mayank Verma, Bhavani Sai Rohit Murkonda, Yoko Asakura, and Atsushi Asakura 11. Isolation, Culture, Functional Assays and Immunofluorescence of Myofiber-Associated Satellite Cells
Thomas O. Vogler, Katherine E. Gadek, Adam B. Cadwallader, Tiffany L. Elston, and Bradley B. Olwin 12. Transplantation-Based Quantitative Assays for Satellite Cell Self-Renewal and Differentiation
Robert W. Arpke and Michael Kyba 13. Non-Invasive Tracking of Quiescent and Activated MuSC Engraftment Dynamics In Vivo
Andrew T.V. Ho and Helen M. Blau 14. Myogenic Progenitors from Mouse Pluripotent Stem Cells for Muscle Regeneration
Alessandro Magli, Tania Incitti, and Rita C.R. Perlingeiro 15. Assaying Human Myogenic Progenitor Cell Activity by Reconstitution of Muscle Fibers and Satellite Cells in Immunodeficient Mice
Maura H. Parker 16. Methods for Mitochondria and Mitophagy Flux Analyses in Stem Cells of Resting and Regenerating Skeletal Muscle
Laura GarcÃa-Prat, Marta MartÃnez-Vicente, and Pura Muñoz-Cánoves
17. Identification, Isolation, and Characterization of Mesenchymal Progenitors in Mouse and Human Skeletal Muscle
Akiyoshi Uezumi, Takehiro Kasai, and Kunihiro Tsuchida
18. FACS Fractionation and Differentiation of Skeletal-Muscle Resident Multipotent Tie2+ Progenitors
Arpita A. Biswas and David J. Goldhamer
Part III Muscle Functional Assays
Justin E. Sperringer and Robert W. Grange 20. In Vivo Assessment of Muscle Contractility in Animal Studies
Shama R. Iyer, Ana P. Valencia, Erick O. Hernández-Ochoa, and Richard M. Lovering
21. Functional Measurement of Respiratory Muscle Motor Behaviors Using Transdiaphragmatic
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