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Nano-patterning di superfici a-C: H per il controllo dell'adsorbimento di proteine
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ISBN10: 6208198372
ISBN13: 9786208198374
Publisher: Edizioni Sapienza
Published: Oct 18 2024
Pages: 104
Weight: 0.36
Height: 0.25 Width: 6.00 Depth: 9.00
Language: Italian
ISBN13: 9786208198374
Publisher: Edizioni Sapienza
Published: Oct 18 2024
Pages: 104
Weight: 0.36
Height: 0.25 Width: 6.00 Depth: 9.00
Language: Italian
Prevedere come le proteine si ripiegano e si adsorbono sulle superfici è un problema complesso di grande rilevanza per i settori della salute e dell'ambiente. Spesso viene esaminato nel contesto più ampio dell'adesione cellulare, ma la comprensione dell'adsorbimento delle proteine è complicata da molti fattori: il rilevamento cellulare e la segnalazione intercellulare, la topografia della superficie e il citoscheletro. Pertanto, gli studi sulle sole proteine sono preziosi per indagare la formazione di biofilm, le malattie da condensazione delle proteine e la tossicità dei nanomateriali. Questo libro presenta un'indagine sistematica sull'adsorbimento di proteine su film sottili di carbonio amorfo idrogenato (a-C: H). La prima metà tratta la preparazione e l'analisi dei pattern di a-C: H mediante fresatura a fascio ionico focalizzato (FIB) e nanoindentazione al microscopio a forza atomica (AFM). La seconda parte studia l'adsorbimento delle proteine per quattro diversi sistemi proteina/solvente. I risultati hanno dimostrato che le superfici modello nano-patternate sono utili per studiare e controllare l'adsorbimento delle proteine, mentre i metodi sviluppati possono essere estesi ad altre tecniche di patterning e a sistemi proteici per studiare in modo indipendente l'influenza della topografia e della composizione sull'adsorbimento delle proteine.
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