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Transgenic Plants: Methods and Protocols
Paperback
Series: Methods in Molecular Biology, Book 847
Botany & HorticultureGeneral ScienceGenetics
ISBN13: 9781493960781
Publisher: Humana
Published: Aug 23 2016
Pages: 497
Weight: 1.95
Height: 1.04 Width: 7.00 Depth: 10.00
Language: English
Contents
Preface
Contributors
Part I. Selection AND DETECTION methods
1. Employment of Cytokinin Vectors for Marker-Free and Backbone-Free Transformation
Craig M. Richael and Caius M. Rommens
2. Organophosphorus Hydrolase; a Multifaceted Plant Genetic Marker Which is Selectable, Scorable, and Quantifiable in Whole Seed
T. Scott Pinkerton, James R. Wild, and John A. Howard
3. Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants
Basel Khraiwesh
Part II. Transformation Technology - Lower Plants
4. Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii
Juliane Neupert, Ning Shao, Yinghong Lu, and Ralph Bock
Part III. Transformation Technology - Rice
5. A High Efficiency Agrobacterium-mediated Transformation System of Rice (Oryza sativa L.)
Kenjirou Ozawa
6. Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene
Masaki Endo, Tsutomu Shimizu and Seiichi Toki
7. Visual Selection in Rice-a Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products
Hiroaki Saika, Haruko Onodera and Seiichi Toki
8. Characterization of Rice Genes using Heterologous a Full-length cDNA Expression System
Mieko Higuchi, Youichi Kondou, Masaki Mori, Takanari Ichikawa, Minami Matsui
9. Bioactive Bead-mediated Transformation of Plants with Large DNA Fragments
Naoki Wada, Joyce A. Cartagena, Naruemon Khemkladngoen, and Kiichi Fukui
Part IV. Transformation Technology - Cereals and Other Monocots
10. Agrobacterium-mediated Transformation of Sorghum bicolor Using Immature Embryos
Songul Gurel, Ekrem Gurel, Tamara I. Miller, and Peggy G. Lemaux
11. Split-transgene Expression in Wheat
Mario Gils, Myroslava Rubtsova, and Katja Kempe
12. Agrobacterium-mediated Transformation of Brachypodium Distachyon
Vera Thole and Philippe Vain
13. Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules
Inger B ksted Holme, Henrik Brinch-Pedersen, Mette Lange and Preben Bach Holm
14. Biolistic Mediated Production of Transgenic Oil Palm
Ghulam Kadir Ahmad Parveez and Bohari Bahariah
15. Transformation of Oil Palm using Agrobacterium tumefaciens
Abang Masli Dayang Izawati, Ghulam Kadir Ahmad Parveez, and Mat Yunus Abdul Masani
Part V. Transformation Technology - Dicots
16. Highly Efficient Transformation Protocol for Plum (Prunus domestica L.)
César Petri, Ralph Scorza, and Chinnathambi Srinivasan
17. Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes
Manjul Dutt, Zhijian T. Li, Sandanand A. Dhekney and Dennis J. Gray
18. Initiation and Transformation of Grapevine Embryogenic Cultures
Sandanand A. Dhekney, Zhijian T. Li, Manjul Dutt, and Dennis J. Gray
19. Development of Highly Efficient Genetic Transformation Protocols for Table Grape Sugraone and Crimson Seedless
Mercedes Dabauza and Leonardo Velasco
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