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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
The contribution of molecular biology to the diagnosis of pneumocystis

The contribution of molecular biology to the diagnosis of pneumocystis

Sessi, Eya
Mtibaa, Latifa
Jemli, Boutheina

Paperback

Medical Reference

ISBN10: 620878686X
ISBN13: 9786208786861
Publisher: Our Knowledge Publishing
Published: Mar 26 2025
Pages: 64
Weight: 0.22
Height: 0.15 Width: 6.00 Depth: 9.00
Language: English
Pneumocystis is an opportunistic infection that mainly affects immature patients. The introduction of molecular biology in the diagnosis of pneumocystis has improved the sensitivity of pathogen detection. The aim of our study is to demonstrate the contribution of real-time PCR compared with staining techniques. Our study is retrospective (January 2022-August 2023), and includes 6 cases of pneumocystis. In 4 cases (67%), co-infections involving Pneumocystis jirovecii were detected in association with HIV, CMV and SARS-CoV-2. Co-infection with Mycobacterium tuberculosis was observed in 1 case (17%). Conventional staining with MGG and Musto-modified Gomori Grocott was negative in all cases. However, real-time PCR analysis showed positive results for all samples, demonstrating 100% sensitivity. Parasite load levels ranged from 10 to106 copies/mL. Quantitative PCR is a highly sensitive technique, with less risk of contamination, and helps clinicians distinguish between colonization and infection.

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