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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Cultivation of BHK-21 cells in adapted serum-free suspension media

Cultivation of BHK-21 cells in adapted serum-free suspension media

Leme, Jaci

Paperback

Biology

ISBN10: 6208659558
ISBN13: 9786208659554
Publisher: Our Knowledge Publishing
Published: Feb 18 2025
Pages: 60
Weight: 0.21
Height: 0.14 Width: 6.00 Depth: 9.00
Language: English
Mammalian cells are the most frequently used hosts for the manufacture of biopharmaceutical proteins and the production of viral vaccines. Quality is a key element in establishing an efficient bioconversion process. In this study, we used the BHK- 21C13 (Baby Hamster Kidney) cell line adapted for suspension cultivation. The use of Fetal Bovine Serum (FBS) is traditionally used and is considered a universal supplement, as it allows growth in various mammalian cell lines; however, the use of FBS presents a risk of prion infection, batch-to-batch variability and an increase in the cost of downstream processing. The aim of this study was to compare the cultivation of BHK-21 C13 cells between two media supplemented with SFB and without SFB, through kinetic studies for static and agitated suspension cultivation using a T-flask, spinner flask and bioreactor, respectively.

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