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Coltivazione di cellule BHK-21 in terreni di sospensione adattati e privi di siero
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ISBN10: 620865954X
ISBN13: 9786208659547
Publisher: Edizioni Sapienza
Published: Feb 18 2025
Pages: 64
Weight: 0.22
Height: 0.15 Width: 6.00 Depth: 9.00
Language: Italian
ISBN13: 9786208659547
Publisher: Edizioni Sapienza
Published: Feb 18 2025
Pages: 64
Weight: 0.22
Height: 0.15 Width: 6.00 Depth: 9.00
Language: Italian
Le cellule di mammifero sono gli ospiti più frequentemente utilizzati per la fabbricazione di proteine biofarmaceutiche e la produzione di vaccini virali. La qualità è un elemento chiave per stabilire un processo di bioconversione efficiente. In questo studio abbiamo utilizzato la linea cellulare BHK- 21C13 (Baby Hamster Kidney) adattata per la coltivazione in sospensione. L'uso di siero fetale bovino (FBS) è tradizionalmente utilizzato ed è considerato un supplemento universale, in quanto consente la crescita di diverse linee cellulari di mammifero; tuttavia, l'uso di FBS presenta un rischio di infezione da prioni, variabilità tra i lotti e un aumento dei costi di lavorazione a valle. Lo scopo di questo studio è stato quello di confrontare la coltivazione di cellule BHK-21 C13 tra due terreni di coltura integrati con SFB e senza SFB, attraverso studi cinetici per la coltivazione in sospensione statica e agitata, utilizzando rispettivamente una fiasca a T, una fiasca a centrifuga e un bioreattore.
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