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Clonage moléculaire, expression et purification d'une nouvelle enzyme lipase
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ISBN10: 6200734127
ISBN13: 9786200734129
Publisher: Editions Notre Savoir
Published: Jul 22 2025
Pages: 104
Weight: 0.33
Height: 0.25 Width: 6.00 Depth: 9.00
Language: French
ISBN13: 9786200734129
Publisher: Editions Notre Savoir
Published: Jul 22 2025
Pages: 104
Weight: 0.33
Height: 0.25 Width: 6.00 Depth: 9.00
Language: French
Shewanella putrefaciens est une bactérie Gram négative. S. putrefaciens est également un anaérobie facultatif capable de réduire le fer et le manganèse en tant qu'accepteur terminal d'électrons dans la chaîne de transport d'électrons, tant dans les milieux solides que liquides. S. putrefaciens est également reconnaissable à sa couleur rose vif. Elle résiste aux conditions environnementales changeantes. Les lipases sont également très répandues chez les bactéries, comme le montrent les recherches d'homologie dans les bases de données protéiques et les génomes bactériens. Des gènes de lipase ont été clonés à partir de champignons et de bactéries, puis leur activité enzymatique a été recherchée. Dans cette étude, nous avons privilégié S. putrefaciens ATCC 8071, car son enzyme lipase n'avait pas encore été caractérisée. Les gènes de lipase de Shewanella putrefaciens ont été transférés dans E. coli à l'aide de techniques de clonage moléculaire. L'enzyme lipase a également été purifiée et caractérisée.
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