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Clonazione molecolare, espressione e purificazione di un nuovo enzima lipasi
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ISBN10: 6200734143
ISBN13: 9786200734143
Publisher: Edizioni Sapienza
Published: Jul 22 2025
Pages: 100
Weight: 0.32
Height: 0.24 Width: 6.00 Depth: 9.00
Language: Italian
ISBN13: 9786200734143
Publisher: Edizioni Sapienza
Published: Jul 22 2025
Pages: 100
Weight: 0.32
Height: 0.24 Width: 6.00 Depth: 9.00
Language: Italian
La Shewanella putrefaciens è un batterio gram-negativo. La S.putrefaciens è anche un anaerobio facoltativo con la capacità di ridurre il ferro e il manganese come accettore terminale di elettroni nella catena di trasporto degli elettroni sia in mezzi solidi che liquidi. La S.putrefaciens è anche riconoscibile per il suo colore rosa brillante. È resistente alle condizioni ambientali mutevoli. Le lipasi sono anche molto diffuse tra i batteri, sulla base di ricerche di omologia nei database delle proteine e nei genomi batterici. I geni della lipasi sono stati clonati da funghi e batteri e poi sono state ricercate le loro attività enzimatiche. In questo studio abbiamo preferito S. putrefaciens ATCC 8071 perché il suo enzima lipasi non era stato caratterizzato in precedenza. I geni della lipasi in Shewanella putrefaciens sono stati trasferiti in E. coli con tecniche di clonazione molecolare. Inoltre, l'enzima lipasi è stato purificato e caratterizzato.
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