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Clonagem molecular, expressão e purificação de uma nova enzima lipase
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ISBN10: 6200734186
ISBN13: 9786200734181
Publisher: Edicoes Nosso Conhecimento
Published: Jul 22 2025
Pages: 100
Weight: 0.32
Height: 0.24 Width: 6.00 Depth: 9.00
Language: Portuguese
ISBN13: 9786200734181
Publisher: Edicoes Nosso Conhecimento
Published: Jul 22 2025
Pages: 100
Weight: 0.32
Height: 0.24 Width: 6.00 Depth: 9.00
Language: Portuguese
A Shewanella putrefaciens é uma bactéria gram-negativa. A S. putrefaciens também é uma bactéria anaeróbica facultativa com a capacidade de reduzir o ferro e o manganês como aceitador terminal de elétrons na cadeia de transporte de elétrons em meios sólidos e lÃquidos. A S. putrefaciens também é reconhecida pela sua cor rosa brilhante. É resistente a condições ambientais variáveis. As lipases também são encontradas amplamente entre as bactérias, com base em pesquisas de homologia em bancos de dados de proteÃnas e genomas bacterianos. Os genes da lipase foram clonados de fungos e bactérias e, em seguida, pesquisados quanto à atividade enzimática. Neste estudo, preferimos S. putrefaciens ATCC 8071 porque a sua enzima lipase não tinha sido caracterizada anteriormente. Os genes da lipase em Shewanella putrefaciens foram transferidos para E. coli com técnicas de clonagem molecular. Além disso, a enzima lipase foi purificada e caracterizada.
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