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Réponse de l'ADN plasmidique chez E. Coli résistant aux médicaments
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ISBN10: 6203898112
ISBN13: 9786203898118
Publisher: Editions Notre Savoir
Published: Apr 22 2025
Pages: 52
Weight: 0.18
Height: 0.12 Width: 6.00 Depth: 9.00
Language: French
ISBN13: 9786203898118
Publisher: Editions Notre Savoir
Published: Apr 22 2025
Pages: 52
Weight: 0.18
Height: 0.12 Width: 6.00 Depth: 9.00
Language: French
La prévalence des E. coli multirésistants (MDR) dans les produits alimentaires (viande de poulet) revêt une grande importance pour la santé publique. La viande de poulet vendue au détail est le principal facteur de propagation des infections à E. coli MDR chez l'homme. Ce livre se concentre sur l'ADN plasmidique d'E. coli MDR, son profilage moléculaire et son traitement. Le profilage moléculaire comprend l'extraction du plasmide par la méthode de l'alcool isoamylique phénol-chloroforme et la détermination du nombre et de la taille des plasmides dans une souche particulière par séparation sur électrophorèse sur gel (0,8 %). Le durcissement des plasmides est effectué en exposant les souches MDR à une concentration sub-inhibitrice d'agents chimiques (SDS) et physiques (chaleur). La confirmation du durcissement du plasmide est effectuée par réplication et antibiogramme. L'objectif est de déterminer la relation entre le plasmide et le profil de résistance aux antibiotiques, c'est-à -dire que la résistance aux antibiotiques est présente soit sur le plasmide (guéri par la guérison du plasmide), soit sur le chromosome.
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