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Resposta do ADN plasmÃdico em E. Coli resistente a múltiplos fármacos
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ISBN10: 620389818X
ISBN13: 9786203898187
Publisher: Edicoes Nosso Conhecimento
Published: Apr 22 2025
Pages: 52
Weight: 0.18
Height: 0.12 Width: 6.00 Depth: 9.00
Language: Portuguese
ISBN13: 9786203898187
Publisher: Edicoes Nosso Conhecimento
Published: Apr 22 2025
Pages: 52
Weight: 0.18
Height: 0.12 Width: 6.00 Depth: 9.00
Language: Portuguese
A prevalência de E. coli multirresistente (MDR) em produtos alimentares (carne de frango) é de grande importância para a saúde pública. A carne de frango vendida a retalho é o principal fator de propagação de infecções por E. coli MDR nos seres humanos. Este livro centra-se no ADN plasmÃdico de E. coli MDR, na sua caraterização molecular e na sua cura. A caraterização molecular inclui a extração do plasmÃdeo através do método de fenol-clorofórmio-isoglicol e a determinação do número e tamanho do plasmÃdeo numa determinada estirpe por separação em eletroforese em gel (0,8%). A cura do plasmÃdeo é efectuada expondo as estirpes MDR a uma concentração sub-inibitória de agentes quÃmicos (SDS) e fÃsicos (calor). A confirmação da cura do plasmÃdeo é efectuada por plaqueamento de réplicas e testes de suscetibilidade a antibióticos. O objetivo é determinar a relação do plasmÃdeo com o padrão de resistência aos antibióticos, ou seja, a resistência aos antibióticos está presente quer no plasmÃdeo (curado pela cura do plasmÃdeo) quer no cromossoma.
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