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Studi tassonomici su Phlebotomus (Larroussius) orientalis del Sudan
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ISBN10: 6209373097
ISBN13: 9786209373091
Publisher: Edizioni Sapienza
Published: Nov 27 2025
Pages: 108
Weight: 0.34
Height: 0.26 Width: 6.00 Depth: 9.00
Language: Italian
ISBN13: 9786209373091
Publisher: Edizioni Sapienza
Published: Nov 27 2025
Pages: 108
Weight: 0.34
Height: 0.26 Width: 6.00 Depth: 9.00
Language: Italian
Questa ricerca è stata condotta per studiare le differenze morfometriche e molecolari nelle popolazioni di Phlebotomus orientalis provenienti da tre diverse regioni del Sudan. Sono stati raccolti campioni di flebotomi Phlebotomine dagli Stati di Gedaref, White Nile e Khartoum. Gli esemplari di P. orientalis sono stati raccolti utilizzando trappole luminose miniaturizzate CDC e trappole adesive in carta. Gli esemplari di flebotomi sono stati quindi montati su vetrini con terreno di Brelese e identificati a livello di specie. I campioni montati di P. orientalis sono stati poi sottoposti a misurazioni morfometriche di 23 e 29 caratteri rispettivamente per le femmine e i maschi. Inoltre, i geni Cyt b, ITS2 rDNA 18S di P. orientalis provenienti da diverse regioni sono stati amplificati mediante PCR convenzionale e in tempo reale. I campioni amplificati dei geni Cyt b e ITS2 sono stati purificati e sequenziati. Le sequenze sono state assemblate, modificate, analizzate e poi confrontate con il database del GenBank. Le popolazioni di P. orientalis provenienti da tre aree geografiche hanno mostrato un certo grado di differenza sia in base all'analisi morfometrica che a quella molecolare.
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