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Sviluppo di biofilm batterici utilizzando E. coli e P. aeruginosa
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ISBN10: 620877277X
ISBN13: 9786208772772
Publisher: Edizioni Sapienza
Published: Mar 23 2025
Pages: 52
Weight: 0.18
Height: 0.12 Width: 6.00 Depth: 9.00
Language: Italian
ISBN13: 9786208772772
Publisher: Edizioni Sapienza
Published: Mar 23 2025
Pages: 52
Weight: 0.18
Height: 0.12 Width: 6.00 Depth: 9.00
Language: Italian
L'esistenza di batteri biofilm sia su superfici naturali che artificiali sta diventando sempre più evidente. I biofilm sono un gruppo complesso di microrganismi, in grado di adattarsi e sopravvivere in un nuovo ambiente, purché vi sia disponibilità di nutrienti, un tempo di incubazione adeguato e un mezzo appropriato. I ceppi batterici di Escherichia coli e Pseudomonas aeruginosa sono stati utilizzati per sviluppare biofilm in laboratorio. Questi ceppi batterici sono stati osservati utilizzando la colorazione di Gram, il test della catalasi e il test dell'ossidasi. Questi ceppi batterici sono stati enumerati per unità di colonie vitali a 0, 2, 4, 6 e 8 ore e incubati rispettivamente a 30 C e 37 C. I ceppi batterici coltivati fino alla fase stazionaria sono stati inoculati in una piastra da microtitolazione a 96 pozzetti a 0, 2, 4, 6 e 8 ore, incubati rispettivamente a 30 C e 37 C, colorati con cristallvioletto, quindi misurati in densità ottica a 540 nm. Il risultato ha mostrato che E. coli e P. aeruginosa sono anaerobi facoltativi Gram-negativi, da entrambi i ceppi batterici sono stati ottenuti biofilm e CPU vitali. L'assorbanza delle cellule aderenti dei ceppi batterici di E. coli e P. aeruginosa è aumentata con il passare del tempo da 0 a 6 ore e diminuita rispettivamente a 8 ore.
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