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Rozwój biofilmów bakteryjnych przy użyciu E.coli i P.aeruginosa
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ISBN10: 6208772788
ISBN13: 9786208772789
Publisher: Wydawnictwo Nasza Wiedza
Published: Mar 23 2025
Pages: 56
Weight: 0.19
Height: 0.13 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786208772789
Publisher: Wydawnictwo Nasza Wiedza
Published: Mar 23 2025
Pages: 56
Weight: 0.19
Height: 0.13 Width: 6.00 Depth: 9.00
Language: Polish
Istnienie bakterii biofilmowych zarówno na naturalnych, jak i sztucznych powierzchniach staje się coraz bardziej oczywiste. Biofilmy są zlożoną grupą mikroorganizmów, zdolną do adaptacji i przetrwania w nowym środowisku pod warunkiem dostępności skladników odżywczych, odpowiedniego czasu inkubacji i odpowiedniego podloża. Szczepy bakterii Escherichia coli i Pseudomonas aeruginosa zostaly wykorzystane do rozwoju biofilmów w laboratorium. Szczepy te obserwowano przy użyciu barwienia metodą Grama, testu katalazy i testu oksydazy. Te szczepy bakterii zostaly policzone pod kątem żywotnych jednostek kolonii po 0, 2, 4, 6 i 8 godzinach i inkubowane odpowiednio w 30 C i 37 C. Szczepy bakteryjne wyrosle do fazy stacjonarnej zaszczepiono na 96-dolkowej plytce mikrotitracyjnej po 0, 2, 4, 6 i 8 godzinach inkubacji odpowiednio w 30 C i 37 C, wybarwiono fioletem krystalicznym, a następnie zmierzono gęstośc optyczną przy 540 nm. Wyniki wykazaly, że E. coli i P. aeruginosa są Gram-ujemnymi fakultatywnymi beztlenowcami, a biofilmy i żywotne CPU uzyskano z obu szczepów bakterii. Absorbancja przylegających komórek szczepów bakteryjnych E. coli i P. aeruginosa wzrastala wraz z uplywem czasu od 0 do 6 godzin i spadala odpowiednio po 8 godzinach.
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