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Leukemia Stem Cells: Methods and Protocols
Hardcover
Series: Methods in Molecular Biology, Book 2185
ISBN13: 9781071608098
Publisher: Humana Pr
Published: Nov 10 2020
Pages: 479
Weight: 2.30
Height: 1.10 Width: 7.70 Depth: 10.30
Language: English
Part I: Introductory Reviews
1. Introduction and Classification of Leukemias
Geoffrey Brown
2. Leukemia Stem Cells: Concept and Implications
Isidro Sánchez-GarcÃa and César Cobaleda
3. Leukemia Stem Cell Drug Discovery
César Cobaleda and Isidro Sánchez-GarcÃa
Part II: Protocols
4. Analysis and Isolation of Mouse Leukemic Stem Cells
Fang Dong, Haitao Bai, and Hideo Ema
5. Mass Cytometry of Hematopoietic Cells
Astraea Jager, Jolanda Sarno, and Kara Davis
6. PCR Technology to Identify Minimal Residual Disease
Giovanni Cazzaniga, Simona Songia, and Andrea Biondi, on behalf of the EuroMRD Working Group
7. Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies
Michaela Kotrova, Nikos Darzentas, Christiane Pott, and Monika Brüggemann, on behalf of the EuroClonality-NGS Working Group
8. Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology)
Daniel Hein, Arndt Borkhardt, and Ute Fischer
9. Single-Cell Transcriptomic Analysis of Hematopoietic Cells
Paulina M. Strzelecka, Anna M. Ranzoni, and Ana Cvejic
10. In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics
Yiran Liang, Thy Truong, Ying Zhu, and Ryan T. Kelly
11. In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation
Kyomi J. Igarashi and Ryo Yamamoto
12. Experimental Competitive Bone Marrow Transplant Assays
Roxann Hétu-Arbour, Sarah Bouali, and Krista M. Heinonen
13. Human T-ALL Xenografts
Patricia Fuentes, MarÃa L. Toribio, and Sara González-GarcÃa
14. An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts
Thalia Vlachou, Marco S. Nobile, Chiara Ronchini, Daniela Besozzi, and Pier Giuseppe Pelicci
15. Leukemic Stem Cell Culture in Cytokine-Free Medium
Xiaolei Liu and Peter S. Klein
16. Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid
Luena Papa, Mansour Djedaini, Manisha Kintali, Christoph Schaniel, and Ronald Hoffman
17. Isolation, Culture, and Manipulation of Human Cord Blood Progenitors
Cristina Priet
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