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Badanie mechanizmów cytotoksycznych w ludzkich komórkach NK
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ISBN10: 6208986036
ISBN13: 9786208986032
Publisher: Wydawnictwo Nasza Wiedza
Published: Jun 20 2025
Pages: 128
Weight: 0.40
Height: 0.30 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786208986032
Publisher: Wydawnictwo Nasza Wiedza
Published: Jun 20 2025
Pages: 128
Weight: 0.40
Height: 0.30 Width: 6.00 Depth: 9.00
Language: Polish
Komórki NK (natural killer) są częścią wrodzonego ukladu odpornościowego. Tworzą one synapsę immunologiczną (IS) z zainfekowanymi lub nowotworowymi komórkami docelowymi i zabijają je za pomocą receptorów śmierci (ligand Fas CD95 i receptor Fas CD178) lub poprzez uwalnianie perforiny i granzymu z granulek litycznych. Wiązanie Fas-FasL indukuje apoptozę. Uwalnianie perforiny może wywolac bezpośrednią lizę komórek docelowych (nekrozę) lub doprowadzic do przeniknięcia granzymów do komórki docelowej, co indukuje apoptozę. Oba rodzaje śmierci komórek docelowych zależne od NK zostaly opisane w literaturze, jednak analizy populacyjne zostaly przeprowadzone wylącznie za pomocą testów końcowych. Dynamiczna regulacja zależnej od NK apoptozy i nekrozy komórek docelowych na poziomie pojedynczych komórek nie zostala jeszcze zbadana. Aby zbadac dynamiczne procesy zachodzące w pojedynczych komórkach, komórki docelowe, które mogą zostac zabite przez ludzkie komórki NK, zostaly stabilnie transfekowane czujnikiem śmierci komórkowej. Test ten pozwala na klasyfikację apoptozy i nekrozy pojedynczych komórek na podstawie różnych wzorów fluorescencji czujnika opartego na FRET.
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