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Investigação de mecanismos citotóxicos em células NK humanas
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ISBN10: 6208986087
ISBN13: 9786208986087
Publisher: Edicoes Nosso Conhecimento
Published: Jun 20 2025
Pages: 128
Weight: 0.40
Height: 0.30 Width: 6.00 Depth: 9.00
Language: Portuguese
ISBN13: 9786208986087
Publisher: Edicoes Nosso Conhecimento
Published: Jun 20 2025
Pages: 128
Weight: 0.40
Height: 0.30 Width: 6.00 Depth: 9.00
Language: Portuguese
As células assassinas naturais (NK) fazem parte do sistema imunitário inato. Elas formam uma sinapse imunológica (IS) com células-alvo infectadas ou tumorigénicas e as matam através de receptores de morte (ligante Fas CD95 e receptor Fas CD178) ou através da libertação de perforina e granzima em grânulos lÃticos. A ligação Fas-FasL induz a apoptose. A libertação de perforina pode provocar a lise direta das células-alvo (necrose) ou levar à penetração de granzimas na célula-alvo, o que induz a apoptose. Ambas as mortes de células-alvo dependentes de NK foram descritas na literatura, mas as análises populacionais foram realizadas apenas através de ensaios de ponto final. A regulação dinâmica da apoptose e necrose dependentes de NK das células-alvo ao nÃvel de células individuais ainda não foi estudada. Para estudar processos dinâmicos de células individuais, as células-alvo que podem ser mortas por células NK humanas foram transfectadas de forma estável com um sensor de morte celular. Este ensaio permite a classificação de apoptoses e necroses de células individuais através de diferentes padrões de fluorescência do sensor baseado em FRET.
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