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Klonowanie TA
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ISBN10: 6208873924
ISBN13: 9786208873929
Publisher: Wydawnictwo Nasza Wiedza
Published: Jul 11 2025
Pages: 60
Weight: 0.21
Height: 0.14 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786208873929
Publisher: Wydawnictwo Nasza Wiedza
Published: Jul 11 2025
Pages: 60
Weight: 0.21
Height: 0.14 Width: 6.00 Depth: 9.00
Language: Polish
Wykorzystaliśmy technikę klonowania TA genu odpowiedzialnego za opornośc na antybiotyki, tj. opornośc na kanamycynę, wykonując podstawowe kroki związane z procesem klonowania. Tlo wynalazku Podstawowym wymogiem skutecznej inżynierii genetycznej bakterii i innych komórek rozmnażanych w kulturach komórkowych jest zdolnośc do selekcji komórek z określoną zmianą genotypową. Najczęstszą strategią selekcji w technologii rekombinacji DNA jest wlączenie markera selekcyjnego do wektora klonującego lub plazmidu. Markerem selekcyjnym może byc sklonowany gen lub sekwencja DNA, która umożliwia oddzielenie komórek gospodarza zawierających marker selekcyjny od tych, które go nie zawierają. Marker selekcyjny wraz z odpowiednią pożywką selekcyjną utrzymuje wektor klonujący w komórkach. W przeciwnym razie, ponieważ replikacja plazmidów jest obciążeniem energetycznym dla gospodarza bakteryjnego, w rosnących kulturach bakterie, które utracily plazmid, mialyby przewagę wzrostu nad komórkami z plazmidem.
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