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Principe de l'analyse par cytométrie de flux
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ISBN10: 6207911040
ISBN13: 9786207911042
Publisher: Editions Notre Savoir
Published: Aug 2 2024
Pages: 72
Weight: 0.26
Height: 0.17 Width: 6.00 Depth: 9.00
Language: French
ISBN13: 9786207911042
Publisher: Editions Notre Savoir
Published: Aug 2 2024
Pages: 72
Weight: 0.26
Height: 0.17 Width: 6.00 Depth: 9.00
Language: French
La cytométrie en flux est une méthode utile pour obtenir le phénotype et les caractéristiques des cellules. Elle est basée sur la diffusion de la lumière par les cellules testées et l'émission de fluorescence par celles-ci. L'émission de fluorescence est obtenue soit par l'utilisation directe de colorants fluorescents, soit par une combinaison de colorants fluorescents et d'anticorps monoclonaux. Ces anticorps conjugués à la fluorescence peuvent détecter et se lier à des molécules de surface ou à des composés internes des cellules, ce qui permet d'identifier des types de cellules dans une population cellulaire diversifiée par cytométrie de flux. Cette méthode a été développée à la base par des immunologistes qui ont essayé de trier des populations pures de cellules les unes des autres et, après les avoir fait proliférer dans un milieu de culture cellulaire, d'étudier le rôle individuel de chaque cellule dans le système immunitaire. De nos jours, l'utilisation des technologies modernes pour augmenter la vitesse et la facilité d'exécution des travaux se développe de plus en plus. La cytométrie en flux est couramment utilisée pour identifier les lignées cellulaires, analyser les tissus cellulaires matures et détecter l'hétérogénéité de la population de cellules tumorales. Au cours de la dernière décennie, l'utilisation de cette méthode dans les laboratoires cliniques et pour le diagnostic de différents types de cancer s'est considérablement accrue.
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