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Principio del test con la citometria a flusso
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ISBN10: 6207911059
ISBN13: 9786207911059
Publisher: Edizioni Sapienza
Published: Aug 2 2024
Pages: 72
Weight: 0.26
Height: 0.17 Width: 6.00 Depth: 9.00
Language: Italian
ISBN13: 9786207911059
Publisher: Edizioni Sapienza
Published: Aug 2 2024
Pages: 72
Weight: 0.26
Height: 0.17 Width: 6.00 Depth: 9.00
Language: Italian
La citometria a flusso è un metodo utile per ottenere il fenotipo e le caratteristiche delle cellule. Si basa sulla diffusione della luce da parte delle cellule in esame e sull'emissione di fluorescenza da parte delle stesse. L'emissione di fluorescenza si ottiene con l'uso diretto di coloranti fluorescenti o con una combinazione di coloranti fluorescenti e anticorpi monoclonali. Questi anticorpi coniugati alla fluorescenza possono rilevare e legarsi a molecole di superficie o a composti interni delle cellule, rendendo possibile l'identificazione di tipi di cellule in una popolazione cellulare diversificata mediante la citometria a flusso. Questo metodo è stato sviluppato fondamentalmente dagli immunologi che hanno cercato di separare popolazioni pure di cellule tra loro e di farle proliferare in un terreno di coltura cellulare per studiare il ruolo individuale di ciascuna cellula nel sistema immunitario. Al giorno d'oggi, l'uso delle moderne tecnologie per aumentare la velocità e la facilità di esecuzione del lavoro si sta espandendo sempre di più. La citometria a flusso è comunemente utilizzata per identificare le linee cellulari, analizzare il tessuto cellulare maturo e rilevare l'eterogeneità della popolazione di cellule tumorali. Nell'ultimo decennio, l'uso di questa metodica nei laboratori clinici e nella diagnosi di vari tipi di tumore è aumentato notevolmente.
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