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Zasada badania za pomocą cytometrii przeplywowej
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ISBN10: 620783707X
ISBN13: 9786207837076
Publisher: Wydawnictwo Nasza Wiedza
Published: May 27 2025
Pages: 60
Weight: 0.21
Height: 0.14 Width: 6.00 Depth: 9.00
Language: Polish
ISBN13: 9786207837076
Publisher: Wydawnictwo Nasza Wiedza
Published: May 27 2025
Pages: 60
Weight: 0.21
Height: 0.14 Width: 6.00 Depth: 9.00
Language: Polish
Cytometria przeplywowa jest użyteczną metodą uzyskiwania fenotypu i charakterystyki komórek. Opiera się ona na rozpraszaniu światla przez badane komórki i emisji przez nie fluorescencji. Emisję fluorescencji uzyskuje się poprzez bezpośrednie użycie barwników fluorescencyjnych lub kombinacji barwników fluorescencyjnych i przeciwcial monoklonalnych. Te przeciwciala sprzężone z fluorescencją mogą wykrywac i wiązac się z cząsteczkami powierzchniowymi lub związkami wewnętrznymi komórek, umożliwiając identyfikację typów komórek w zróżnicowanej populacji komórek za pomocą cytometrii przeplywowej. Metoda ta zostala zasadniczo opracowana przez immunologów, którzy próbowali sortowac czyste populacje komórek od siebie i po namnożeniu ich w pożywce do hodowli komórkowej w celu zbadania indywidualnej roli każdej komórki w ukladzie odpornościowym. Obecnie coraz częściej wykorzystuje się nowoczesne technologie w celu zwiększenia szybkości i latwości wykonywania pracy. Cytometria przeplywowa jest powszechnie stosowana do identyfikacji linii komórkowej, analizy dojrzalej tkanki komórkowej i wykrywania heterogeniczności w populacji komórek nowotworowych. W ostatniej dekadzie wykorzystanie tej metody w laboratoriach klinicznych i diagnostyce różnych typów nowotworów dramatycznie wzroslo.
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