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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Hydrocarbon and Lipid Microbiology Protocols: Biochemical Methods

Hydrocarbon and Lipid Microbiology Protocols: Biochemical Methods

McGenity, Terry J.
Timmis, Kenneth N.
Nogales, Balbina

Hardcover

Series: Springer Protocols Handbooks

Medical ReferenceTechnology & EngineeringBiology

ISBN10: 3662491354
ISBN13: 9783662491355
Publisher: Springer
Published: Sep 7 2016
Pages: 188
Weight: 1.25
Height: 0.50 Width: 7.00 Depth: 10.00
Language: English
This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds. They include methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates. Protocols for the isolation and biochemical analysis of lipids and polyhydroxyalkanoates, food storage products made during nutrient abundance that represent important biotechnological products, are presented. The extraction of membrane lipid rafts, sub-organelles that fulfil important functional roles for the cell membrane, and the isolation and characterisation of membrane phospholipid biomarkers, are also described. The purification and characterisation of integral membrane hydrocarbon-oxidising enzymes are addressed. Lastly, two generic methods for the genetic analysis of catabolic pathways and analysis of ligand binding are presented.

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