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  • Open Daily: 10am - 10pm
    Alley-side Pickup: 10am - 7pm

    3038 Hennepin Ave Minneapolis, MN
    612-822-4611

Open Daily: 10am - 10pm | Alley-side Pickup: 10am - 7pm
3038 Hennepin Ave Minneapolis, MN
612-822-4611
Hydrocarbon and Lipid Microbiology Protocols: Genetic, Genomic and System Analyses of Communities

Hydrocarbon and Lipid Microbiology Protocols: Genetic, Genomic and System Analyses of Communities

McGenity, Terry J.
Timmis, Kenneth N.
Nogales, Balbina

Paperback

Series: Springer Protocols Handbooks

Medical ReferenceTechnology & EngineeringBiology

ISBN10: 3662570629
ISBN13: 9783662570623
Publisher: Springer
Published: Apr 30 2018
Pages: 288
Weight: 1.15
Height: 0.63 Width: 7.00 Depth: 10.00
Language: English
This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. Methods are described for the creation and bioinformatic assessment of metagenomic and metatranscriptomic libraries, and for metaproteomic analyses, which provide important insights into the metabolic potential and interactions of community members. These in turn lead to specific hypotheses concerning the functional contributions of individual populations in the community, which may be investigated by the stable isotope probing approaches described in this Volume, making it possible to identify those community members primarily responsible for particular functions. Methods for the direct extraction of proteins from environmental samples for sequencing and activity tests are presented, providing a broad overview of prevailing metabolic activities and of the types of microbe involved in them. Protocols for the analysis of nutrient flow through microbial communities and for the modelling of dynamic physiological interactions in communities are also provided. Lastly, the book presents a protocol for the quantitative assessment of permissiveness for the transfer of conjugative plasmids, important agents of physiological change and evolution in microbial communities.

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